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Protein and
polyphenol profile changes in soybean roots under aluminum
stress
Dechassa Duressa*, Khairy Soliman
and Ernst Cebert
Department of Natural Resources and Environmental Sciences, Alabama A&M University, Normal, AL 35762, USA.
*Corresponding author. E-mail:
dech_1@yahoo.com.
Accepted 6 April, 2010 |
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It is well documented that aluminum (Al) toxicity is the
most important constraint of crop production on acid soils.
Chelation of Al in the rhizosphere with root secreted
organic acid anions is a common mechanism of Al tolerance in
most plants including soybean. Phenolic substances are
recently implicated as additional physiological mechanism of
plant Al tolerance. This study was undertaken to investigate
the role of phenolics in soybean Al tolerance and to examine
protein expression changes in soybean roots under Al stress.
An Al-tolerant soybean genotype PI 416937 and Al-sensitive
Young were used in the study. Protein and polyphenol profile
changes in response to Al stress, were examined in roots
tips of hydroponically grown plants 72 h post treatment. Al
significantly increased total phenol exudation from roots of
the Al tolerant genotype PI 416937, whereas, flavonoid
content did not vary with treatment. Al also altered the
expression level of several proteins in genotypic and non
genotypic specific manner. This is the first study to show
that polyphenol is involved in soybean Al tolerance. Future
research should consider quantification of individual
flavonoid compounds in root tissue as well as culture
solution, and sequencing and functional annotation of Al
regulated proteins.
Key words:
Soybean, aluminum tolerance, protein expression, phenolics. |
