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Full Length Research paper
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Multi-drug resistant Staphylococcus aureus in
clinical cases in Ile-Ife, Southwest Nigeria
Adegoke, Anthony
Ayodeji1* and Komolafe, Amos Omoniyi2
1Department
of Microbiology, University of Uyo, Uyo, Akwa Ibom State,
Nigeria.
2Department
of Medical Microbiology and Parasitology, Obafemi Awolowo
University Hospitals Complex, Ile-Ife, Nigeria.
*Corresponding author. E-mail:
anthonyadegoke@yahoo.co.uk. Tel: 2348038398510.
Accepted 26, February 2009 |
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Abstract |
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A study to investigate the susceptibility pattern of
Staphylococcus aureus to conventional antibiotics being
frequently prescribed in Ile-Ife, South Western Nigeria was
carried out. One hundred and seven samples from various clinical
samples were collected from Obafemi Awolowo University Teaching
Hospitals Complex, Ile-Ife, Nigeria. The isolates of S.
aureus were characterized and identified using standard
microbiological techniques. The isolates harvested were
subjected to in-vitro antibiotic susceptibility tests
using the disc diffusion method and beta-lactamase test was
carried out using starch paper hydrolysis. One hundred and five
isolates of S. aureus were harvested. The in vitro
susceptibility of the bacterial isolates to antibiotics
indicated 75.3-92.5% sensitivity to cefotaxime, ciprofloxacin
and pefloxacin. However, they were 65% producers of beta
lactamase while 45-72% resistant to penicillin, ampicillin,
tetracycline and cotrimoxazole. Out of the 56 isolates tested
with methicillin, 28 (50%) were resistant while 18 (32.1%) were
sensitive. It was observed 70% of the tested isolates were beta
lactamase producers. Multiple resistance was observed to 10
frequently prescribed antibiotics in the area. This suggests
possible abuse of these drugs, poor hospital attendance and the
need for better enlightenment campaign against the use of drug
without prescription.
Key words:
Staphylococcus aureus,
ciprofloxacin, multiple antibiotic resistant index, beta
lactamase test. |
