Effects of ovariectomy and estradiol valerate or progesterone on serum insulin level in rats

Ahmadi R.¹* and Oryan S. H.²

¹Department of Experimental Science, Faculty of Sciences, Islamic Azad University, Hamedan Branch, Iran.

²Department of Biology, Faculty of Sciences, Tarbiat Moallem University,

Tehran, Iran.

*Corresponding author. E-mail: rahahmadi2001@yahoo.com.

Accepted 14 April, 2009

   Abstract

Experimental data from in vitro studies suggest that insulin and sex hormones interact. The main purpose of the present study was to examine the effects of progesterone and estradiol on serum insulin in female rats. Seven week old female albino (Wistar) rats were used in our study. Progesterone (20 mg/kg/day) or estradiol valerate (200 µg/kg/day) were injected subcutaneously in ovariectomised and non-ovariectomised rats. After 4 weeks, serum insulin level was measured indicating decreased serum insulin level in ovariectomised rats compared with control group (P<0.01). In contrast to progesterone replacement, decreasing of serum insulin in ovariectomised rats was prevented by estradiol replacement (P<0.05). Serum insulin level was also increased in estradiol receiving and decreased in progesterone receiving non-ovariectomised rats (P<0.001). Conclusively, our findings indicated that estradiol was serum insulin enhancer hormone and progesterone was serum insulin reducer hormone in rats.

 Key words: Insulin, ovariectomy, progesterone, estradiol, rat.