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Iron
starvation induces expression of a putative xylanase gene
in
Salmonella enterica subsp. enterica
serovar Enteritidis mini-transposon5 lacZ1 mutants
Kazhila C. Chinsembu* and André Faul
Department of Biological Sciences, Faculty of Science,
University of Namibia, P/Bag 13301, Windhoek, Namibia.
*Corresponding author. E-mail:
kchinsembu2008@yahoo.co.uk,
kchinsembu@unam.na. Tel: 264-61-2063426. Fax:
264-61-2063791.
Accepted 30 June, 2010. |
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In this study,
Salmonella enterica
subsp. enterica serovar Enteritidis mini-transposon5
lacZ1 mutant strains induced under iron starvation were
analyzed. Inverse polymerase chain reaction (IPCR) was used
to isolate iron starvation-induced DNA fragments upstream of
mini-Tn5 (containing a promoter-less lacZ
gene) insertion in S. enterica subsp.
enterica serovar Enteritidis mutants showing β-galactosidase
activity during growth in Fe3+-deprived media.
Out of ten mutant strains analyzed, four (Ez188, Ez477,
Ez1819 and Ez2508) were induced during growth in the
presence of the iron chelator, 2,2’-dipyridyl. IPCR products
of Ez188 and Ez2508 were reamplified by nested PCR. Sequence
analysis of the Ez188 PCR product revealed that a putative
xylanase gene was induced under iron starvation conditions.
Key words: Salmonella mutants, iron starvation,
induced, genes, putative xylanase.
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