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Rapid detection of
Salmonella species in newborne calves by polymerase
chain reaction
I. M. Moussa1*, M. H. Ashgan2, M.
S. Mohamed3, K. H. F. Mohamed4 and A.
A. Al-Doss1
1Center of Excellence in Biotechnology, King Saud University, P. O. Box
2460 Riyadh, King Saudi Arabia.
2College of Applied Studies and Community Service, King Saud University,
P. O. Box 2460 Riyadh, King Saudi Arabia.
3Department of Microbiology, Riyadh Colleges for Dentistry and Pharmacy,
Riyadh, King Saudi Arabia.
4Department of Microbiology, Faculty of Veterinary Medicine, Cairo
University, Cairo, Egypt.
*Corresponding author. E-mail:
imoussa1@ksu.edu.sa
, moussaihab@gmail.com
Accepted
5 March, 2010 |
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Fecal
samples collected from 85 diarrheic calves and 65 apparently
healthy contact calves were examined for the presence of
Salmonella species using bacteriological examination and
fimA gene amplification assay (PCR). Salmonella
were isolated from 43.53% of diarrheic calves and from
27.69% of apparently healthy contact calves. Salmonella
typhimurium were isolated from diarrheic and contact
calves in percentages of 17.65 and 15.38% respectively;
whereas Salmonella entertidis were isolated in
percentages of 11.76 and 7.69%, respectively. Salmonella
dublin were isolated from the diarrheic calves in a
percentage of (8.24%) and contact calves in a percentage of
(4.62%), but Salmonella anatum were isolated
from diarrheic calves only in a percentage of (5.88%), The
sensitivity and specificity of PCR were 100 and 95.8%,
respectively during examination of fecal samples obtained
from diarrheic calves, while during the examination of those
obtained from contact calves the percentages were 100 and
89.4%, respectively.
Key
words:
Salmonella, fimA gene, diarrheic calves,
Salmonella typhimurium, Salmonella entertidis. |
