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Full Length Research
Paper
Arbuscular mycorrhiza
contribution to the growth performance and heavy metal
uptake of Helianthus annuus LINN in pot culture
O. O. Awotoye1, M. B. Adewole*1, A.
O. Salami2 and M. O. Ohiembor1
1Institute of Ecology and Environmental Studies, Obafemi Awolowo
University, Ile-Ife, Nigeria.
2Department of Crop Production and Protection, Obafemi Awolowo University,
Ile-Ife, Nigeria.
*Corresponding author. E-mail:
adewoledele2005@yahoo.co.uk
Tel: +234 8034784267.
Accepted 12 June, 2009
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Abstract |
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A greenhouse
experiment was conducted to assess the arbuscular mycorrhiza
contribution to the growth performance and heavy metals (Cd and
Pb) uptake of Helianthus annuus L. from polluted soils.
Cadmium sulfate (CdSO4.8H2O) and lead
acetate ((CH3COO)2Pb.3H2O) were
applied at the concentrations: Cd- 0,20,40,60,80 mg kg-1
and Pb-0,250,500,750,1000 mg kg-1 in 90 plastic pots
filled with 5 kg of topsoil each. Propagules of Glomus
mosseae (GM) and Glomus intraradices (GI) at 25 g per
pot were applied. Non-inoculated pots served as controls. Each
treatment was replicated thrice in completely randomized design.
Data were analysed using ANOVA and descriptive statistics.
Results showed that arbuscular mycorrhiza (AM) fungi
significantly (p ≤ 0.05) influenced the number of leaves, plant
height and stem girth of sunflower plants only at 80 mg Cd kg-1
concentration. Highest values of 0.11 mg Cd kg-1 and
0.29 mg Pb kg-1 were obtained with GI in the dry
shoot of Helianthus annuus while 0.05 mg Cd kg-1
and 0.23 mg Pb kg-1 were remediated with GM
application. Least values of Cd and Pb were removed when no AM
was applied. GM and GI fungi enhanced the growth of
Helianthus annuus and the uptake of Cd and Pb; but GI
performed better. Also, a significant (p < 0.05) reduction of AM
fungi colonization percentage was obtained with increase in Cd
and Pb concentrations.
Key words:
Glomus mosseae, Glomus intraradices, Helianthus
annuus, phytoremediation, polluted soil. |
