Antimicrobial and antioxidant activities of essential oil and methanol extract of Jasminum sambac from Djibouti

Fatouma Abdoul-Latif^{1, 2}, Prosper Edou³*, François Eba³, Nabil Mohamed⁴, Adwa Ali⁴, Samatar Djama⁴, Louis-Clément Obame^5,6, Ismael Bassolé⁵ and Mamoudou Dicko²

¹Laboratoire de Biologie, Université de Djibouti, BP 1904 Djibouti, République de Djibouti.

²Laboratoire BAEBIB, Université de Ouagadougou 03, BP 7021 Ouagadougou, Burkina Faso.

³Ecole Normale Supérieure de Libreville, Laboratoire Pluridisciplinaire des Sciences, BP 17009 Libreville, Gabon.

⁴Centre d' Etudes et de Recherches de Djibouti BP 486 Djibouti, République de Djibouti.

⁵Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles, Laboratoire de Microbiologie et de Biotechnologie, Université de Ouagadougou 03, BP 7131 Ouagadougou, Burkina Faso.

⁶Laboratoire de Substances Naturelles et de Synthèses Organométalliques (LASNSOM), Université des Sciences et Techniques de Masuku, BP 943, Franceville, Gabon.

*Corresponding author. E-mail: edouengonga@yahoo.fr.

Accepted 8 January, 2010.

   Abstract

The essential oil was subjected to screening for their possible antioxidant activity by two complementary test systems, namely DPPH free radical scavenging and β-carotene-linoleic acid assays. Butylated hydroxytoluene (BHT) was used as positive control in both test systems. In the DPPH test system, the IC₅₀ value of essential oil and methanol extract were respectively 7.43 and 2.30 µg/ml. In the β-carotene-linoleic acid system, oxidation was effectively inhibited by Jasminum sambac, the RAA value of essential oil and methanol extract were respectively 96.6 and 93.9%. When compared to BHT, the oil and methanol extract were nearly the same value. Furthermore, the essential oil and methanol extract were evaluated for its antimicrobial activity using disc diffusion and microdilution methods. The essential oil and methanol extract showed better activity against bacterial species than against yeast.

Key words: Jasminum sambac, antimicrobial activity, antioxidant activity, essential oil, methanol extract.