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A comparative study of
morphological and molecular diversity in Ethiopian lentil (Lens
culinaris Medikus) landraces
Edossa Fikiru1*, Kassahun Tesfaye2
and Endashaw Bekele2
1Mada
Walabu University, P. O. Box 84, Bale-Robe, Ethiopia.
2Department
of Biology, Faculty of Science, Addis Ababa University, P.
O. Box 1176, Addis Ababa, Ethiopia.
*Corresponding author. E-mail:
edossa.fikiru@yahoo.com,
edossa.fikiru@gmail.com.
Accepted May 31, 2010. |
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Genetic diversity assessment with different methods and
their comparison could provide complementary information for
improvement and conservation programs. However, such
information is not available for Ethiopian lentil landraces.
To fill this gap, nine morphological traits and four ISSR
primers were used on seventy Ethiopian lentil landrace
accessions to study morphological and genetic diversity
among the accessions, assess the correlation between
phenotypic and genetic dissimilarities, and classify the
accessions into groups based on both markers. Analysis of
variance of morphological data showed significant (p < 0.01)
differences among accessions for all traits. However, days
to maturity and flowering, seed weight and plant height
showed low phenotypic coefficient of variation. From
principal component (PC) analysis number of seeds and pods,
seed yield and number of secondary branch were included in
PC1, whereas, days to maturity and flowering were dominant
in PC2. Seed weight and number of primary branch were also
dominant in PC3 and PC4, respectively. With regard to
molecular analysis, accessions from Gonder, Shewa and Wello
(with =
0.203, 0.174 and 0.162, respectively) showed moderate level
of diversity. The average and overall diversity index
values, respectively, were 0.145 and 0.264. The overall
morphological and genetic dissimilarities ranged from 0.825
- 11.018 and 0.000 - 0.340 with average value of 3.921 and
0.125, respectively. The correlation ( )
between morphological and genetic dissimilarity matrices was
0.28 (p < 0.001) indicating that either of the markers could
be used for genetic diversity study. Cluster analysis based
on morphological and molecular markers, respectively,
revealed two and three major groups of lentil landrace
accessions. The result of the present study would be useful
for further collection and conservation, and improvement
programs.
Key words:
Clustering, correlation, genetic diversity, ISSR, lentil
landraces, morphological variation, principal component.
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