Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos

Nadeem Ahmad Siddique¹, Mohd Mujeeb¹*, Abdul Kalam Najmi² and Mohd Akram³

¹Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) New Delhi-110062, India.

²Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) New Delhi-110062, India.

³Department of preventive and social medicine, Faculty of medicine, Jamia Hamdard (Hamdard University) New Delhi-110062, India.

*Corresponding author. E-mail: mujeeb_zaidi@yahoo.co.in.

Accepted 5 of August, 2009

   Abstract

In existing study, we carried out an efficient record of the comparative antioxidant activity in methanolic extract of the selected parts (leaves, root and stem bark) of Aegle marmelos. Total content of phenol and flavonoid was quantitatively estimated in different parts of A. marmelos. The total phenolic contents varied from 9.8367 ± 0.0235 to 1.7281 ± 0.049 mg g^-1. Total flavonoid contents were between 8.248 ± 0.029 to 1.087 ± 0.002 mg g^-1. Free radical scavenging activity of different extracts was evaluated by using DPPH (1, 1 -Diphenyl- 2 -picryl hydrazyl) method. The highest free radical scavenging effect was observed in leaves with IC₅₀ = 2.096µg ml ^–1. The effectiveness of radical scavenging activity of leaves extract was about 10 times greater than reference antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to more powerful radical scavenging effect as shown by methanolic extract of A. marmelos leaves. 

Key words: Aegle marmelos, antioxidant, flavonoids, phenols, 1, 1 –diphenyl - 2 -picryl hydrazyl.