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Morphological
evaluation of olive plants propagated in vitro
culture through axillary buds and somatic embryogenesis
methods
Leva Annarita
Istituto per la Valorizzazione Del Legno e delle
Specie Arboree, CNR Polo Scientifico via Madonna Del Piano,
10 Sesto F.no (Firenze), Italy. E-mail:
leva@ivalsa.cnr.it.
Tel.:
+39 0555225684. Fax: +39 0555225656.
Accepted 16 of March, 2009
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The morphological fidelity of the olive plants propagated
through axillary buds, microplants and somatic
embryogenesis, somatic plants was evaluated. Thirty-two
morphological traits were used to characterize the tissue
culture propagated olive plants. The microplants showed very
high phenotypic similarity compared to plants produced by
conventional cutting propagation method.
The somatic plants exhibited variant morphological stable
phenotypes, among somaclonal population two variant
phenotypes were studied: BOS (bush-olive somaclone) and COS
(columnar-olive somaclone). A wide range of plant traits
were differently involved in somaclonal variation as plant
height, canopy dimensions, leaf, inflorescence and fruit
dimensions in respect to the putative control plants. The
present study has established that the morphological
stability of tissue culture-derived olive plants is strictly
related with the in vitro propagation method used.
Key words:
Tissue culture, phenotypic stability, somaclonal variation,
Olea europaea. |
