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Characterization of callus
formation in leaf of Euphorbia helioscopia
Zi-Song Yang1,3,
Guang-Deng Chen2,3*,
Yun-Xiang Li2,3 and Jiao Chen2,3
1Department
of Chemistry and Life Science, Aba Teachers College, Pixian,
611741, People’s Republic of China.
2College
of Life Science, China West Normal University, Nanchong,
637009, People’s Republic of China.
3Key
Laboratory of Southwest China Wildlife Resources
Conservation (China West Normal University), Ministry of
Education, Nanchong, 637009, People’s Republic of China.
*Corresponding author. E-mail:
gdengchen@yahoo.com .
Accepted
11 June, 2009 |
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The callus was induced on Murashige and Skoog’s (MS) medium
supplemented with 2,4-dichlo-rophenoxyacetic acid (2,4-D) or
6-Benzylaminopurine (BAP) from the Chinese medicinal herb
Euphorbia helioscopia in the family Euphorbiaceae. The
highest frequencies of callus induction
were observed on MS medium supplemented with
3.0 mg l-1 2,4-D. Callus (yellow, loose,
granular) was chosen as research focus in the long-term
callus maintenance, the proliferation coefficient could
still hold about 10 after subcultured 5 turns. Histological
analysis was done to reveal the developmental pattern of
callus formation. Detailed analysis revealed induction of
leaf yielded many endogenous eumeristematic cells which were
still embedded in the parenchymatic tissue.
Key
words:
Euphorbia helioscopia,
callus induction, callus maintenance, histological,
developmental pattern. |
