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Full Length
Research Paper
Protective effect of quercetin against necrosis and
apoptosis induced by experimental ischemia and reperfusion
in rat liver
khaki Arash
Department of Veterinary Pathology, Islamic Azad
University Tabriz Branch, Iran. E-mail:
arashkhaki@yahoo.com
Accepted
29 December, 2009
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Quercetin is a well-known flavonoid and a strong
antioxidant. Quercetin an important flavonoid possesses
beneficial effects on health due to its antioxidant
function. One mechanism of the antioxidant action of
quercetin was involved in scavenging free radicals, such as
superoxide radicals generated by xanthine and xanthine
oxidase. To study the effect of quercetin on apoptosis and
necrosis induced by 1 h ischemia followed by 1.5 h
reperfusion. Adult Wistar rats underwent 1 h of partial
liver ischemia followed by 1.5 h reperfusion. Eighteen
Wistar rats were divided into sham-operated control group
(I) (n = 10), ischemia and reperfusion (I/R) group (0.9 %
saline (5 ml/kg, orally) for 14 days) (II) (n = 10), and
quercetin group (15 mg/kg body weight daily orally for 14
days before inducing ischemia-reperfusion maneuver) (III)(n=10).
Apoptotic and necrotic hepatocytes, nitric oxide levels in
hepatocytes. Liver injury was assessed by plasma alanine
transaminases (ALT), aspartate transaminases (AST), liver
histopathology. An ischemic and reperfusion hepatocellular
injury occurred as was indicated by increased serum ALT,
AST, histopathology. Pretreatment with quercetin
significantly decreased serum ALT and AST level and
apoptotic and necrotic cells after 1 h ischemia followed by
3 h of reperfusion. Nitric oxide production in hepatocytes
was increased twofold by quercetin treatment when compared
with I/R group. Histopathology studies showed markedly
diminished hepatocellular injury in quercetin -pretreated
rats during the hepatic I/R. Thus, it may be concluded that
quercetin can significant protection from necrosis and
apoptosis in I/R injury with nitric oxide and plasma alanine
transaminases (ALT) production and it has anti-ROS effect in
ischemic reperfusion.
Key
words: Apoptosis,
ischemia, reperfusion, quercetin. |
