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Full Length
Research Paper
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In vitro evaluation of the influence of
compounds with tricyclic structure on the microbial growth
and expression of virulence factors
Cristina Delcaru1,
Mariana Carmen Chifiriuc3*, Dracea Olguta1,
Carmen Iordache1,
Carmen Limban2, George
Mihai Nitulescu2, Missir Alexandru Vasile2,
Ileana Cornelia Chirita2, Carmellina Badiceanu2,
Coralia Bleotu3, Robert Sakizlian4 and
Anca Michaela Israil1
1National
Institute of Research and Development for Microbiology and
Immunology Cantacuzino,
Spl. Independentei 103, cod 060631, Bucharest, Romania.
2Pharmaceutical
Chemistry Department, Faculty of Pharmacy, “Carol Davila”
University of Medicine and Pharmacy, Traian Vuia 6, sect. 2,
020956, Bucharest, Romania.
3Faculty
of Biology, University of Bucharest, Aleea Portocalelor 1-3,
Bucharest, Romania.
4Department
of Physical Education and Sport, University of Bucharest,
Bucharest, Romania.
*Corresponding author. E-mail:
carmen_balotescu@yahoo.com.
Accepted 11 May, 2012 |
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Abstract |
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The purpose of this study was to evaluate the in vitro
antimicrobial activity of some newly synthesized oxepins and to
investigate their influence on the expression of virulence
factors of Gram-negative (Escherichia coli, Proteus
vulgaris, Morganella morganii, Salmonella arizonae,
Klebsiella planticola, and Pseudomonas aeruginosa)
and Gram-positive (Staphylococcus aureus) strains.
Our results demonstrated that out of the 48 tested compounds, 24
exhibited specific antimicrobial activity, the lowest minimal
inhibitory concentration (MIC, 62.5 µg/ml) being noticed against
E. coli, K. planticola, S. arizonae and
P. aeruginosa strains. When tested for the expression of the
virulence factors, by comparison with the controls, the strains
grown in the presence of subinhibitory concentrations of the
tested antimicrobial substances exhibited only slight
differences versus the control strain, concerning the adherence
patterns and indexes and the expression of some pore-forming
toxins.
The flow cytometry assay revealed that
the active compounds did not exhibit toxic activity against HeLa
cells at the working concentration.
Key words:
Antimicrobial activity, virulence factors expression, new
oxepins.
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