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Gas chromatography – mass
spectrometry analysis of the hexane extract of
Calliandra portoricensis and its antimicrobial activity
A. T. Orishadipe*, J. I. Okogun and E. Mishelia
National
Institute for Pharmaceutical Research and Development, P. M.
B. 21, Idu Industrial Estate, Idu, Abuja FCT, Nigeria.
*Corresponding author. E-mail:
oorisat@yahoo.com. Tel:
234-805-874-3077.
Accepted
21 June, 2010 |
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Calliandra
portoriscensis is widely used in traditional medicine preparation
in South western part of Nigeria for the management of sickle cell
crisis and other health challenges. Analysis of the hexane extract of
C. portoricensis by GC-MS revealed 14 – methyl
methylpentadecanoate as the major component. Other fatty acids/fatty
acid methyl esters detected were hexadecanoic acid, methylhexadecanooate,
9 – oxo – methyl nonanoate and some other minor components. The extract
was also investigated for preliminary antimicrobial activity using the
following pathogenic bacteria Escherichia coli, Staphyloccocus aureus,
Salmonella gallinallum, Klebsiela pneumonia, Bacillus subtilis
and Pseudomonas aeruginosa. The extract was found to be active
against S. aureus, E. coli and S. gallinallum, on the other hand
it was not active against K. pneumonia, B. subtilis and P.
aeruginosa. The results of the GC-MS analysis and the biological
assay are discussed.
Key words:
Calliandra portoricensis, gas chromatography, mass
spectrometry, antimicrobial, fatty acid methyl esters. |
