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African Journal of
Microbiology Research Vol. 1 (6), 092-099,
November, 2007
ISSN 1996-0808 © 2007 Academic Journals
Full Length
Research Paper
In vitro screening of antibacterial
activity of aqueous and alcoholic extracts of
various Indian plant species against selected
pathogens from Enterobacteriaceae
Parekh J and Chanda S*
Phytochemical, Pharmacological and
Microbiological Laboratory,Department of
Biosciences, Saurashtra University, Rajkot, 360
005,Gujarat, India.
*Corresponding author. E-mail:
sumitrachanda@yahoo.com.
Accepted 6 November, 2007
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Thirty four medicinal plants,
belonging to twenty eight different families,
were screened for potential antibacterial
activity against six bacterial strains
belonging to Enterobacteriaceae, viz.
Enterobacter aerogenes ATCC13048,
Escherichia coli ATCC25922, Klebsiella
pneumoniae NCIM2719, Proteus mirabilis
NCIM 2241, Proteus vulgaris NCTC8313,
and Salmonella typhimurium ATCC23564.
Antibacterial activity of aqueous and alcoholic
extracts was tested by the agar disc diffusion
and agar well diffusion methods. The
ethanol/methanol extracts were more active than
aqueous extracts for all the plants studied. The
most susceptible bacterium was K. pneumoniae,
while the most resistant bacteria were S.
typhimurium and E. coli. From the
screening experiment, Woodfordia fruticosa
Kurz.
showed best antibacterial activity. Hence, this
plant may be used further to isolate and
evaluate the therapeutic antimicrobials.
Key words:
Medicinal plants, antibacterial activity,
aqueous extracts, alcoholic extracts,
Enterobacteriaceae |
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