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Extracellular trehalose
production by Micrococcus lylae and its enhancement
by heat shock
Myung-Ji Seo1*, Wook-Dong Kim2 and
Soon-Ok Kim3
1Fermentation
and Functionality Research Group, Korea Food Research
Institute, Sungnam 463-746, Republic of Korea.
2Division
of Bio·New
Drug Development, Central Research Institute, Chem Tech
Research Incorporation (C-TRI), Namyangju 472-030, Republic
of Korea.
3Division
of Research and Product Development, Union Korea Pharm., Co.
Ltd., Seoul 138-878, Republic of Korea.
*Corresponding
author. E-mail: mjseo@kfri.re.kr. Tel: +82-31-780-9362. Fax:
+82-31-709-9876.
Accepted 30 May, 2012 |
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We examined the effects of heat (50°C),
osmotic (3% NaCl), and solvent (3% ethanol) shock on the
extracellular trehalose production by Micrococcus lylae
KCTC 3517 in glucose-supplemented medium. Cultivation of
M. lylae at 37°C
for 12 h followed by heat shock of exponentially growing
cells
at 50°C
for 2 h at 12 h-intervals resulted in maximum trehalose
production (1.72 mg/ml), which was 2-fold higher than that
in untreated cells (0.85 mg/ml). Simple activated carbon
column chromatography was then used to purify trehalose,
which was eluted as major compound using 10-15% ethanol.
Finally, the concentrated eluent contained moderately pure
trehalose.
Key words:
Trehalose, Micrococcus lylae, heat shock. |
