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Cloning and phylogenetic analysis of
bovine leukemia virus gag gene in Iranian isolate
Hassan Momtaz
Department of Microbiology,
Faculty of Veterinary Medicine, Islamic Azad University of
Shahrekord branch, Shahrekord, Iran. P. O. Box.166.
E-mail:
hamomtaz@yahoo.com.
Tel: 0098 381 3361083.
Accepted 22 December, 2009 |
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Bovine leukemia virus (BLV)
is a member of the family Retroviridae, genus
Deltaretrovirus that has three important gene including
gag, pol and env. This virus causes B-cell lymphocytosis and
lymphosarcoma in cows. In the first step PCR product of
gag gene of BLV isolated in different regions of Iran
and BLV-FLK strain was cloned into a pTZ57R/T vector, then
insert were digested by BglII and XhoI
restriction enzymes and cloned into pET-28(a) as an
expression vector. Analysis of the partial bovine leukemia
virus (BLV) virus gag gene sequences obtained from
insert and was carried out. The Iranian BLV gag sequence was
compared to five other corresponding sequences of BLV
isolated in different countries. Nucleotide analyzing of the
sequences were shown a variation of 1 - 8.7% and
constructing phylogenetic tree revealed two clusters in it.
First cluster included New York, Maryland, Japan and Iranian
sample, second cluster included Australian and Argentina
strains. Iranian isolates were significantly identical to
European and American isolates.
Key words:
Bovine leukemia virus, p24 protein, pTZ57R/T vector,
pET-28(a) vector, phylogenetic analysis. |
