|
Clinical isolating outer membrane protein pattern from Avian
Escherichia coli of China
Zhang Tie*, Wang Chun-guang and Zhao Xing-hua
College of Traditional Chinese Veterinary Medicine, Hebei
Agricultural University, Dingzhou 073000, China.
*Corresponding author. E-mail:
zhangtie1998@163.com.
Tel: +86-312-2381803
Accepted 13 July, 2010 |
|
The outer membrane protein (OMP) were extracted from38
strains of Avian Escherichia coli which were isolated
from the dead chickens of chicken breeding farms in 3 areas
of Baoding, Qinhuangdao and Beijing by using ultrasonic
cleaving and N- Lauroyl sarcosine sodium and OMP typing was
done by SDS- PAGE to understand the genetic relationship of
isolated strains from Avian pathogenic E. coli. There
were 3 OMP types in these 38 strains of E. coli, in
which, type OMP-1 was in common for 6 serotypes of isolated
strains of O78, O88, O2, O18,
O93 and O76, type OMP-abelonged
to isolated strain of O76, OMP-b
was found from all of the isolated strains of O93,
O78, O88, O2, this
indicated the identical serotype strain might belong to
quite different OMP patterns and among isolated strains
without any relationship in serotype could have the same OMP
type. Among isolated strains with the same serotype strains
might have genetic differentiation. And among the isolated
strains of different serotypes, there might be the genetic
relationship with different degree.
Key words:
Avian Escherichia coli, outer membrane protein, O
serotypes. |
