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Screening of potential infants’ lactobacilli isolates
for amino acids production
Tarek, M. El-Nemr1 and Hesham E. Mostafa2*
1Department
of Dairy Science and Technology, Faculty of Agriculture,
Alexandria University, Egypt.
2Bioprocess
Development department, Genetic Engineering and
Biotechnology Research Institute, Mubarak City for
Scientific Research, Research Area-Borg El-Arab, Post Code
21934, Alexandria, Egypt.
*Corresponding
author. E-mail:
hesham@yahoo.com. Tel: +1 780 632 8225. Fax: +1 780 632
8385.
Accepted 15 October, 2009 |
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A total of 9
lactic acid bacteria (LAB) isolated from Egyptian infants,
belonging to lactobacilli strains, were screened and
selected according to their amino acids production. The
isolate strains produced different essential amino acids in
fermentation of M17 medium. Lactobacillus
strain L1 produced the highest yield of histidine 92.5 mg/l,
L2 strain produced the highest amount of asparagine 59.61
mg/l, cysteine 32.96 mg/l, phenylalanine 17.49 mg/l and
tyrosine
244.91 mg/l. On the other hand,
Lactobacillus
strain L3 is the most potential culture for the production
of citrulline 61.12 mg/l and glutamic 68.78 mg/l.
This strain also
produced the
highest amount of lysine, ornithine and proline, which were
57.04, 95.58 and 87.06 mg/l, respectively. While the strain
L4 produced the highest amount of glycine 32.12 mg/l, strain
L7 produced the highest amount of alanine 147.55 mg/l and
strain L5 produced the highest amount of
asparatic acid
218.09 mg/l. The present study concluded that the
Lactobacillus strains have the potential to produce
different amino acids with variance concentrations.
Key words:
Lactobacilli, Lactobacillus, amino acids, screening,
production. |
