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Screening and optimization of nutrients for L-asparaginase
production by Bacillus cereus MNTG-7 in SmF by
plackett-burmann design
M. Sunitha*, P. Ellaiah and R. Bhavani Devi
Pharmaceutical
Biotechnology Division, Department of Pharmaceutical
Sciences, Andhra University, Visakhapatnam 530003, India.
*Corresponding
author. E-mail:
suneet_rahul@rediffmail.com
Accepted 17 December, 2009 |
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The medium
components influencing the enzyme production were screened
using Plackett-Burman design. A total of 67 nutrients
comprising of fifteen each of carbon, nitrogen, mineral/salt
and eleven each of inorganic nitrogen sources and amino acid
sources were screened for the production of L-asparaginase
by SmF. This design involves screening of up to ‘n-1’
variables in just ‘n’ number of experiments. Regression
coefficients and t-values were calculated by subjecting the
experimental data to statistical analysis. Among the 67
nutrients based on their performance in terms of product
yield and cost, tapioca starch, L-asparagine, ammonium
oxalate, gelatin and CaCO3 were identified as
most effective and therefore selected for inclusion in
surface methodology studies.
Key words:
L-asparaginase, nutritional factors, submerged fermentation,
Bacillus cereus MNTG-7. |
