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The impact of bacteriophages in bacteria removal associated
with soba stabilisation station efficiency
Ayman Ahmed Elshayeb
Alneelain
University Faculty of Science and Technology School of
Biotechnology, Zip 11111, Postal code 11121 Box 12702.
Khartoum – Sudan.
E-mail:
ayman_elshayeb@yahoo.com.
Tel: +
249122974208.
Accepted 16 October, 2009 |
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The existence
of bacteriophages in wastewater of Soba Stabilisation
Station was determined by isolating and identifying methods
for their activities against Escherichia coli and
Staphylococcus aureus isolated from the anaerobic,
facultative and maturation ponds. The general viable count
of the bacteria showed an average of 2.0 x 106
cfu/ml. In
broth media the affection of the bacteriophage interactions
with bacteria showed increasing of bacteriophages with
concomitant decrease in bacteria due to culture clearance,
where the readings of the turbidity for the first and second
infection showed statistical significant of light
transmission among E. coli
phages’ samples due to place of sample collections as
follows: from the anaerobic and facultative ponds P > 0.05,
facultative and maturation P < 0.05 and anaerobic and
maturation P > 0.05. Whilst, the S. aureus phages
samples’ light transmission from the anaerobic and
facultative P < 0.05, facultative and maturation P < 0.05
and anaerobic and maturation P > 0.05. On solid media, the
affection of the bacteriophage was recognised by the phage
plaque formation on bacterial cultures. The linear equations
of phages’ densities and distributions according to their
wavelength were y = 0.0008x + 0.0303 for E. coli
phage and y = -0.0102x + 0.2438 for
S. aureus phage. This study concluded that phages
naturally present where their hosts present and naturally
destroyed bacteria that aided to recover from polluted
environment.
Key words:
Bacteriophage, Escherichia coli/ general viable
count, light transmission, linear equations, stabilisation
station, Staphylococcus aureus. |
