|
Antimicrobial resistant pattern of Escherichia coli
from human clinical samples in Osogbo, south western Nigeria
Olowe O. A1,
Okanlawon B. M2, Olowe R. A3 and
Olayemi A. B4
1Department
of Medical Microbiology and Parasitology, P.M.B.4400, Ladoke
Akintola University of Technology, College of Health
Sciences, Osogbo, Osun State, Nigeria.
2Department of Biomedical Sciences, Faculty of
Basic Medical Sciences, Ladoke Akintola University of
Technology, College of Health Sciences, Osogbo, Osun State,
Nigeria.
3Department of Biology, Federal University of
Technology, Akure Ondo State, Nigeria.
4Department of Microbiology, Faculty of Sciences,
University of Ilorin, Kwara State, Nigeria.
*Corresponding author. E-mail:
olowekunle@yahoo.com.
Tel: +4915204937219
Accepted 17 January, 2008
|
|
We screened 211 clinical samples of which total of 135
Escherichia coli isolates from different human
clinical specimens comprising urine, stool, wound swabs,
high vaginal swabs, ear swabs and blood obtained from
patients at Ladoke Akintola University Teaching Hospital,
Osogbo, Osun State, Nigeria. The isolated E. coli
were screened for their antibiograms and plasmid profiles.
Seven antimicrobial drugs were used during the study. The
prevalence of strains resistance to antimicrobials were;
Tetracycline (91.6%), Ampicillin (86.7%), Sulphnamide
(77.8%) and Gentamicin and Nalidixic acid which were (39.3%)
and (4.1%) respectively. A total of seven antibiotic
resistance profiles were obtained with over 64% of the
isolates showing multi-drug resistance. Plasmids of three
size ranges were detected in all of the isolates. Isolates
with high multi-drug resistance profiles were found to
possess multiple plasmids with large sizes in the range < 6
– 25 kb. Very large resistance levels > 85% were detected
against Tetracycline, Sulphnamide, and Cotrimoxazole while
Nalidixic acid showed least resistance of 4.1% among the
isolates. Majority of the isolates were positive for
betalactamase production when subjected to starch paper
method.
Key word: Escherichia coli, betalactamse,
antibacteria resistance. |
