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Effect of Gongronema
latifolium crude leaf extract on some cardiac enzymes of
alloxan-induced diabetic rats
E. E. Edet1, M. I. Akpanabiatu2*
A. E. Eno3, I. B. Umoh1 and E. H. Itam1
1Department of Biochemistry, Faculty of Basic Medical Sciences, College of
Medical Sciences,
University
of Calabar, P. M. B. 1115,Calabar - Nigeria
2Biochemistry Department, Faculty of Basic Medical Sciences, College of
Health Sciences, University of Uyo, P. M. B. 1017, Uyo -
Nigeria.
3Physiology Department, Faculty of Basic Medical Sciences, College of
Basic Medical Sciences, University of Calabar, P. M. B.
1115, Calabar - Nigeria.
*Corresponding author. E-mail:
akpanabiatu@yahoo.com.
Accepted 7 October, 2009 |
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The effect
of Gongronema latifolium on serum cardiac enzymes in
alloxan induced diabetic rat models and normal control rats
were determined using graded doses of 80% ethanol extract of
G. latifolium leaf. The serum activities of aspartate
aminotransferase (AST), creatine kinase (CK), creatine
kinase isoenzyme (CKMB) and lactate dehydrogenase (LD) were
determined in diabetic Wistar albino rats; diabetic control
and normal Wistar albino rats after 2 weeks administration
of G. latifolium leaf extract. Serum CKMB and LD
activities of all the experimental groups significantly (p ≤
0.001) increased in diabetic rats when compared to
non-diabetic rats. CK and LD decreased significantly in
diabetic and non-diabetic rats treated with G. latifolium
leaf extract when compared with the control. Equally, CKMB
and LD of diabetic rats treated with G. latifolium
leaf extract decreased significantly except CKMB of group 4
and LD of group 3 animals. There were significant (p ≤
0.001) decreased activity of serum AST in diabetic rats when
compared to the non-diabetic rats. However, AST did not
change significantly in non-diabetic rats while in diabetic
rats AST increased significantly (p ≤ 0.001) when compared
with their respective controls. These data suggest that the
effects of G. latifolium leaf extract at our
concentrations are not dose dependent and hepatotoxic. The
extract of this plant is likely to be of biological
significance in cardiovascular complication of diabetic and
non-diabetic users.
Key words:
Alloxan-induced diabetes, Gongronema latifolium, cardiac enzymes,
myocardial infarction. |
