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A study on the combined
administration of chloroquine and insulin effect on serum
transferases and phosphatases activity during increased
dietary fat and calcium consumption
Ajani E. O.*, Salau B. A., Ezima E. N. , Oyefuga O. H.
and Osineye P. O.
Department
of Biochemistry, Faculty of Basic Medical Sciences, Olabisi
Onabanjo University, Remo Campus, Ikenne, Ogun State,
Nigeria.
*Corresponding author. E-mail:
immanbisi@yahoo.com. Tel:
08055533192.
Accepted 28
December, 2008 |
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Previous
studies reported on relationship between chloroquine
administration, insulin and glucose homeostasis, but the
role of dietary fat and the combined effect of the drugs on
liver function have not been exploited. The present study
was set up to investigate the effect of combined
administration of insulin and chloroquine on serum
transferase and phosphates activity during increased dietary
fat and calcium consumption. Thirty (30) adult male albino
rats randomly assigned into five (5) groups were used in the
study. Group A was placed on normal diet (normal control); B
(test control) C, D, and E were placed on an increased
dietary fat and calcium. Drugs were administered as follows:
group C, insulin (100 µg/kg body weight once daily); group
D, chloroquine (Imarsel Chemical Co. Ltd., Chinon, Hungary,
15 mg/kg body weight) thrice weekly over a period of 12
weeks, and group E, chloroquine combined with insulin. All
rats were sacrificed after 15 weeks treatment period. Blood
was withdrawn and activities of the enzymes were determined.
Combined administration of the drugs increased serum alanine
amino transferase, aspartate transaminase and alkaline
phosphate activity and decreased acid phosphatase activity.
The result suggests that combined administration of insulin
and chloroquine may result in hepatic injury particularly in
individual whose dietary regimen includes increased fat and
calcium.
Key
words: Chloroquine, insulin, liver function,
transaminases, phosphatases, dietary calcium, fat. |
