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Allethrin-induced
biochemical changes and properties of human erythrocyte
membrane
Narendra M., Kavitha G., Padmavathi P., Helah Kiranmai A.
and Varadacharyulu N. C.*
Department
of Biochemistry, Sri Krishnadevaraya University, Anantapur -
515 003, Andhra Pradesh, India.
*Corresponding author; e-mail:
nchvaradacharyulu@yahoo.com. Phone: 91 (+) 08554
273337(R) cell: (0) 9866157337
Accepted 20
August 2007
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Erythrocyte membranes from twelve human volunteers exposed
regularly to allethrin, a mosquito repellent of type-I
pyrethroid, were analyzed for cholesterol (C), phospholipids
(P), and individual phospholipid classes to assess changes
induced by this toxicant. A decrease in C and P moieties
with no change in C: P ratio was observed with allethrin
exposure. A significant reduction in the amount of
phosphotidyl serine (PS) was noticed indicating that PS is
an allethrin sensitive phospholipid species. Furthermore,
decreased red cell membrane lipid peroxidation (LPO) and
with no change in osmotic haemolysis of erythrocyte was
observed. Increased plasma and red cell nitrate and nitrite
were evident suggesting that the bioavailability of nitric
oxide may have rendered tolerance to erythrocyte membrane by
protecting the cell from haemolysis and oxidative damage due
to its free radical scavenging and antioxidant effects.
Keywords: Allethrin, nitric oxide, osmotic haemolysis,
phosphatidyl serine, rbc biochemical changes.
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