|
Comparative study on the
determination of assay for laccase of Trametes sp.
Airong Li, Yue Zhu, Liang Xu, Wenqing Zhu and Xingjun
Tian*
School of
Life Science,
Nanjing University, Nanjing 210093, P. R. China.
*Corresponding author.
E-mail: tianxj@nju.edu.cn.
Telephone and Fax: +86-25-83686787
Accepted 14
Aug 2008 |
|
The aim of this
work was to determine the sensitivity among compounds in common
use for detecting laccase activity. In this study, three assay
procedures to measure laccase activity of Trametes sp.
were performed in Kirk’s basal salts mediums of three dyes. In
the assay methods, three substrates were employed, which were
2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), ο-dianisidine
and guaiacol. The results indicate that laccase activity used
ABTS as a substrate was significantly higher than the other two.
Therefore, the ABTS method is recommended while detecting
laccase activity.
Key words: ABTS, ο-dianisidine, guaiacol, laccase
activity, Trametes sp. |
