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AQY1
gene from Debaryomyces hansenii
Juan Carlos González-Hernández
Facultad de Ciencias Médicas y Biológicas “Dr. Ignacio
Chávez”, Universidad Michoacana de San Nicolás de Hidalgo,
Morelia, Michoacán, México.
E-mail:
jcgh1974@yahoo.com.
Tel: +52 433 312 05 10. Ext: 120. Fax: +52 433 312 00 14.
Ext: 132.
Accepted
15 April, 2010. |
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Aquaporins are members of the major intrinsic protein
superfamily of integral membrane proteins that enable the
transport of water, glycerol and other solutes across
membranes in diverse organisms. In yeasts, the proposed
physiological roles of aquaporins are related to their
contribution to freeze tolerance, osmoregulation and water
transport. This article reports a contribution to the freeze
tolerance in the heterologous expression of the
Debaryomyces hansenii aquaporin gene in Saccharomyces
cerevisiae. In the present experiments, the transformant
cells were similarly sensitive to osmotic stress conditions,
since their growth capacity phenotype was equivalently
abolished in the presence of 0.6 and 1.0 M NaCl. Northern
analysis of the yeasts studied revealed a correlation
between freeze resistance and the aquaporin gene AQY1.
Key
words:
Debaryomyces hansenii,
Saccharomyces cerevisiae, aquaporin, freeze
tolerance.
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