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Full
Length Research Paper
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Ginger extract
(Zingiber
officinale)
triggers apoptosis and
G0/G1
cells arrest in
HCT 116 and
HT 29 colon
cancer cell lines
Shailah Abdullah1, Siti
Amalina Zainal Abidin1, Noor Azian Murad2,
Suzana Makpol1, Wan Zurinah Wan Ngah1
and Yasmin Anum Mohd Yusof1*
1Department
of Biochemistry, Faculty of Medicine, Universiti Kebangsaan
Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur,
Malaysia.
2Centre
of Lipids and Engineering and Applied Research, Universiti
Teknologi Malaysia, Jalan Semarak, 50300 Kuala Lumpur,
Malaysia.
*Corresponding author.
E-mail: anum@medic.ukm.my.
Tel: 603-4040 5297.
Fax: 603-2693 8037.
Accepted 16
April, 2010. |
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Abstract |
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Although many studies have shown the antitumor properties of
ginger extract (Zingiber officinale), little is known
regarding the mechanism of its effects. This study was
conducted to determine the mechanism of antitumor effects of
ginger extract by evaluating apoptosis rate and cell cycle
progression status in colon cancer cell lines HCT 116 and
p53 defective HT 29. HCT 116 and HT 29 cells were cultured
in the presence of ginger extract at various concentrations
for 24 h. The percentage of cell viability was determined by
3-(4, 5-dimethylthiazol-2-yl)-2, 5-di phenyl tetrazolium
bromide (MTT) assay. Our results showed that ginger extract
inhibited proliferation of HCT 116 and HT 29 cells with an
IC50 of 496 ± 34.2 μg/ml and 455 ± 18.6 μg/ml,
respectively. We also found that ginger extract at
increasing concentrations induced apoptosis dose dependently
in both colon cancer cells. Apoptosis rates were 11.15,
35.05 and 57.49% for HCT 116 and 4.39, 19.81 and 28.09% for
HT 29 at 200, 500 and 800 μg/ml of ginger extract,
respectively. Ginger extract arrested HCT 116 and HT 29
cells at G0/G1 and G2/M
phases with corresponding decreased in S-phase. This study
suggests that ginger extract may exert its antitumor effects
on colon cancer cells by suppressing its growth, arresting
the G0/G1-phase, reducing DNA
synthesis and inducing apoptosis.
Key words:
Zingiber officinale, HCT 116, HT 29, G0/G1
phase, S phase, apoptosis. |
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