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Standardized alcoholic
extract of Phyllanthus fraternus exerts potential
action against disturbed biochemical parameters in
diabetic animals
Munish Garg1*, Chanchal Garg2, V.
J. Dhar3 and A. N. Kalia3
1Department of
Pharmaceutical Sciences, Maharshi Dayanand University,
Rohtak-124001, Haryana, India.
2Department of
Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard,
New Delhi-110062, India.
3Department of Pharmacognosy, I. S. F. College of Pharmacy, Moga-142001,
Punjab, India.
*Corresponding author. E-mail:
mgarg2006@gmail.com.
Tel: 09812588857.
Accepted 14 June, 2010 |
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Alcoholic extract of
Phyllanthus fraternus Webster whole plant (PFAE)
prepared by successive solvent treatment was administered at
a dose of 500 mg/kg body weight once in a day for 21 days
to the alloxan induced diabetic albino rats. Certain
biochemical parameters that is lipid profile (total
cholesterol, high density lipoprotein, triacylglycerols),
kidney functions (urea, creatinine) and liver functions
(alkaline phosphate, alanine aminotransferase, aspartate
aminotransferase) were evaluated and compared with normal
and standard drug tolbutamide (200 mg/kg body weight)
administered group. As a result, drug treatment has
significantly improved the disturbed biochemical parameters
at variable degrees when compared with standard drug. The
phytochemical studies conducted for standardization of the
extract showed the presence of tannins and flavonoids as
major phytoconstituents. The total phenolics content was
found to be 37.51 mg/g of drug extract. Quantitative
estimation carried out on two major flavonoids by HPTLC
confirmed a concentration of 1.706% w/w rutin and 5.614% w/w
of quercetin present in the alcoholic extract. In
conclusion, owing to the positive potential activity against
disturbed biochemical parameters associated with diabetes,
P. fraternus
can be used effectively in the management of this deadly
disease.
Key words:
Phyllanthus fraternus, renal functions, liver functions
tests, lipid profile, total phenolics.
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