Table 2. PCR oligonucleotide resources.

Oligonucléotides pour la PCR

Calculation of melting point of a oligonucleotide.

http://www.citi2.fr/bio2/OligoTM.html

Oligonucleotide properties calculator

Prediction of melting temperature.

http://www.basic.nwu.edu/biotools/oligocalc.html

http://www.microbiology.adelaide.edu.au/learn/oligcalc.htm

Oligonucleotide analyzer

Generates Tm, free energy, molecular weight and hairpin and dimer formation structures.

http://www.rnature.com/oligonucleotide.html

 

Oligo Tm Determination

Prediction of Tm.

http://alces.med.umn.edu/rawtm.html

Poland

Prediction of melting temperatures of primers.

http://www.biophys.uni-duesseldorf.de/local/POLAND/poland.html

PROLIGO

Oligos parameter calculation.

http://www.gensetoligos.com/Calculation/calculation.html