Table 3. Screening of Musa clones for BSV with monoclonal and polyclonal antibodies by TAS-ELISA.
|
Musa Clone |
Symptom |
aMean absorbance value (n=3) at 405 nm at
1h substrate incubation time. bm.s.e. = +
0.015 |
|||
|
BSV 3F9/1 Monoclonal Antibody |
BSV 3D4/2 Monoclonal Antibody |
BSV Mouse antiserum |
|||
|
FHIA 1 |
Symptomless |
0.073 |
0.065 |
0.091 |
|
|
FHIA 2 |
Symptomless |
c0.333 |
0.199 |
0.287 |
|
|
FHIA 3 |
Symptomless |
0.483 |
0.137 |
0.315 |
|
|
SH 3363 |
Yellow streak |
0.077 |
0.066 |
0.08 |
|
|
SH 3436 |
Symptomless |
0.071 |
0.069 |
0.087 |
|
|
SH 3640 |
Yellow streaks |
0.99 |
0.294 |
0.501 |
|
|
TMPx 548-4 |
Yellow streaks |
1.337 |
0.423 |
0.983 |
|
|
TMPx 548-9 |
Yellow streaks |
0.438 |
0.194 |
0.4 |
|
|
TMPx 548-9 |
Yellow streaks |
1.696 |
0.655 |
1.479 |
|
|
TMPx 582-4 |
Yellow streaks |
0.592 |
0.235 |
0.493 |
|
|
TMPx 1112-1 |
Yellow streaks |
1.18 |
0.335 |
0.892 |
|
|
TMPx 1658-4 |
Yellow streaks |
1.939 |
0.831 |
1.236 |
|
|
TMPx 26378 |
Yellow streaks |
0.654 |
0.364 |
0.615 |
|
|
TMPx 2796-5 |
Yellow streaks |
0.519 |
0.233 |
0.479 |
|
|
TMPx 4698-1 |
Symptomless |
0.068 |
0.086 |
0.07 |
|
|
TMPx 4698-1 |
Yellow streaks |
0.768 |
0.21 |
0.659 |
|
|
TMPx 5511-2 |
Yellow streaks |
1.24 |
0.339 |
0.938 |
|
|
TMPx 5511-2 |
Symptomless |
0.066 |
0.067 |
0.089 |
|
|
TMPx 7002-1 |
Yellow streaks |
1.123 |
0.299 |
0.705 |
|
|
TMPx 7152-2 |
Symptomless |
0.44 |
0.163 |
0.37 |
|
|
TM 3x 26388 |
Yellow streaks |
0.782 |
0.306 |
0.725 |
|
|
TM 3x 26388 |
Yellow streaks |
0.81 |
0.246 |
0.801 |
|
|
TM 3x 26636 No 2 |
Yellow streaks |
0.084 |
0.072 |
0.088 |
|
|
TMB2x 5105-1 |
Yellow streaks |
0.412 |
0.196 |
0.396 |
|
|
Cardaba |
Yellow streak |
0.068 |
0.061 |
0.071 |
|
|
Lakanau |
Yellow streaks |
0.077 |
0.072 |
0.08 |
|
|
BSV infected plantain |
Yellow streaks |
1.876 |
0.542 |
1.251 |
|
|
Healthy plantain |
Symptomless |
0.061 |
0.06 |
0.062 |
|
aMean
ELISA reading that was at least
twice that of healthy plantain leaf (0.062)
was indicative of the presence of BSV, and as such considered as a positive test
for BSV.
bm.s.e.=
Mean standard error.