African Journal of Biotechnology Vol. 2 (6), pp. 153–156, June 2003   

ISSN 1684-5315  © 2003 Academic Journals

Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD)

Bahy Ahmed Ali

Nucleic Acid Research Dept., Genetic Engineering & Biotechnology Research Institute (GEBRI), Mubarak City For Scientific Research & Technology Applications, Alexandria, Egypt. E-mail: bahyali@hotmail.com   or bahyali@arabia.com,   Fax: 203 4593323

Accepted 12 May 2003

RAPD technique was used in this study to detect DNA band variations between both normal and abnormal male of broiler chicken based on RAPD marker. DNA polymorphisms between normal and mutant birds were detected using fifteen oligonucleiotide primers. Using these primers, DNA band loss ranged from 25 to 75%. Data demonstrated that RAPD marker could detect DNA alterations.

Keywords: DNA alteration, RAPD, abnormal phenotype, male, broiler chicken.