African Journal of Biotechnology Vol. 2 (7), pp. 185–188, July 2003   

ISSN 1684-5315  © 2003 Academic Journals

Full Length Research Paper

Analysis of AVR4 promoter by sequential response-element deletion                

Olukosi, YA¹ and Iwalokun, BA²*

¹Genetics Division, Nigerian Institute of Medical Research (NIMR), 6, Edmond Crescent, Yaba – Lagos, PMB 2013, Lagos - Nigeria.

*Corresponding author: E-mail: bamwal@yahoo.com

Accepted 24 June 2003

Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2_907-176, Avr4pBLCAT2_809-176, Avr4pBLCAT2_789-176, Avr4pBLCAT2_429-176 and Avr4pBLCAT2_302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities.

Key words: Avidin, AVR4 promoter, chloramphenicol acetyl transferase.