African Journal of Biotechnology
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African Journal of Biotechnology Vol. 2 (12), pp. 683-691, December 2003 ISSN 1684-5315 © 2003 Academic Journals
Review Thin Cell Layer technology in ornamental plant
micropropagation and biotechnology
Jaime
A. Teixeira da Silva Faculty
of Agriculture, Kagawa University, Miki-cho, Ikenobe, 2393, Kagawa-ken,
761-0795, Japan. Telfax: +81 (0)72 726 8178. E-mail: jaimetex@lycos.com. Accepted 4 December 2003 |
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| Abstract | |||||
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Thin cell
layer (TCL) technology originated almost 30 years ago with the controlled
development of flowers, roots, shoots and somatic embryos on tobacco
pedicel longitudinal TCLs. Since then TCLs have been successfully used in
the micropropagation of many ornamental plant species whose previous in
vitro regeneration was not successful using conventional methods. This
review examines the fundamentals behind TCLs, and their application in
ornamental plant micropropagation and transformation. Key words: Chrysanthemum, lily, somatic embryogenesis, thin cell layer, tobacco, transformation.
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