African Journal of Biotechnology Vol. 2 (8), pp. 251-253, August 2003

ISSN 1684-5315  © 2003 Academic Journals

Short Communication

Isolation of total DNA from bacteria and yeast

Marco Ligozzi* and Roberta Fontana

Dipartimento di Patologia, Sezione di Microbiologia Università degli studi di Verona

Corresponding author; Phone: 0039-45-8027601, fax: 0039-45-584606, e-mail: marco.ligozzi@univr.it

Accepted 15 July 2003

Many procedures in molecular biology require the isolation of high quality genomic DNA. This study investigated a new method to extract DNA from Gram-negative, Gram-positive bacteria, Mycobacteria and yeasts. Guanidine thioisocyanate present in DNAzol is capable of binding DNA to silica particle column. Subsequently the silica with adsorbed DNA is washed to remove impurities and the clean DNA eluted in appropriate buffer. Results indicated that the new extraction method is simple and reproducible. This isolation technique is faster and easier to perform than the other conventional extraction methods. Finally the recovered DNA is of high quality and suitable for downstream applications.

Key words: DNAzol, DNA isolation, bacteria, yeast.