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The establishment of a
slow-growth conservation system in vitro for two wild
lily species
Du Yun-peng, Li Wen-yuan, Zhang Ming-fang, He Heng-bin,
and Jia Gui-xia*
College of Landscape Architecture, National Engineering
Research Center for Flowers, Beijing Forestry University,
Beijing 100083, P. R. China.
*Corresponding author. E-mail:
gxjia@bjfu.edu.cn.
Tel:
+86 135 2161 5634.
Accepted 15 December, 2011 |
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There are abundant resources of wild lily in China. To
achieve a sustainable use for these resources, a
slow-growing preservation system should be well established.
Therefore, to get an optimal storage condition, tube
seedlings of Lilium davidii and Lilium longiflorum
were used as explants cultured on nine different media
at 24 and -2°C separately, which included: 1/4 or full
Murashige and Skoog medium (MS) with 3, 6 or 9%
sucrose,
1/4 or 1/2 MS with 1.0 or 3.0 mg/L abscisic acid (ABA).
During the storage, the spout
growth,
and multiplication rate were measured every month. Results
show that the growth restriction degrees for both species
were related to the four factors (MS content, the
concentration of
sucrose
and ABA, temperature). The inhibitory effect was more
obvious at -2°C. At 24°C, the medium should be replaced
every three to four months; otherwise the contamination rate
would rise with the extension of storage time. At -2°C, the
1/4 MS with 9% sucrose or 3.0 mg/L ABA (M3A) were more
effective, especially the latter one. The M3A played the
greatest inhibitory effect on height growth of the two lily
species,
on which
both
species had been conserved on the original medium for more
than 15 months. The tube seedlings conserved for 15 months
could turn to normal plantlets after re-growth for one month
which showed no obvious difference in morphology. 87.5%
plantlets planted in peat could survive. In conclusion, the
promising conservation condition was M3A at -2°C which was
suitable for mid-long term preservation of lily germplasm
resources.
Key words:
Lily, wild species, slow-growth, conservation. |
