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Induction of
somatic embryogenesis and plant regeneration in the
reed grass (Phragmites
communis Trin.)
Jeong-Eun Lee1, Sang-Gyu
Seo1, Bong-kyu Kim1, Seong-Min Woo2,
Bon-Cheol Koo3, Tae-Ho Park4, Yong Pyo
Lim and Sun-Hyung Kim1*
1Department of
Environmental Horticulture, The University of Seoul,
Jeonnong-dong 90, Dongdaemun-gu, Seoul 130–743, South Korea.
2Phygen Inc., Bongmyeong-dong
692-6, Yuseong-gu, Daejeon 305-301, South Korea.
3Bioenergy Crop Research
Center, National Institute of Crop Science, RDA, Muan,
Jeonnam 534-833, South Korea.
4Department of
Horticulture, Daegu University, Jillyang, Gyeongsan 712-714,
South Korea.
*Corresponding author.
E-mail: mongdal@uos.ac.kr.
Tel:
+82-02-2210-5728. Fax: +82-02-2210-2838.
Accepted 8 August, 2011 |
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An in vitro culture system for the large-scale
propagation of Phragmites communis Trin.
(reed) was established by optimizing culture
conditions for callus induction and differentiation together
with plant
propagation using
regenerated plantlets. Callus was induced from stem segments
with callus induction medium containing auxin:
4-fluorophenoxyacetic acid (4-FA) or 2,4-dichlorophenoxy
acetic acid (2,4-D). A high
frequency of callus induction was observed at relatively low
concentrations (0.5 and 1 mg L-1) of both 2,4-D
or 4-FA. However, high concentrations (3, 4 and 5 mg L-1)
of either auxin suppressed callus induction. When applied
for the first time, 1.0 mg L-1
4-FA markedly improved the frequency of callus
induction (up to 93%). The callus was then transferred to MS
medium supplemented with 1.0 mg L-1 2,4-D to
promote the formation of embryogenic calli. The calli were
grown in MS supplemented with different concentrations of
2,4-D and naphthaleneacetic acid (NAA) alone or in
combination with benzyladenine (BA). After seven weeks of
culture, the regeneration efficiency was determined for the
calli maintained for the 45 differentiation media
formulations. The highest regeneration capacity was obtained
from the medium containing 0.05 mg L-1 NAA and 2
mg L-1 BA, and the combination of 0.2 mg L-1
NAA and 2 mg L-1 BA. Propagation of the
regenerated plantlets was also examined in medium containing
different sucrose concentrations; this experiment found that
60 g L-1 sucrose showed the best growth rate.
These improved regeneration and propagation systems could be
used for bioreactor-based mass propagation or an
in vitro culture system, and would be useful for
transformation in Phragmites communis Trin.
Key words:
Phragmites communis Trin, callus,
regeneration, propagation, in vitro culture,
2,4-dichlorophenoxy acetic acid, 4-fluorophenoxyacetic acid. |
