Full Length Research Paper

Molecular study and phylogenetic analysis of Mycoplasma synoviae isolated from poultry flocks from Mazandran Province of Iran

Hosseini Aliabad S. A.¹*, Pourbakhsh, S. A.², Charkhkar S.³, Bozorgmehri Fard M. H.⁴ and Shikhi, N.³

¹Veterinary Department, Faculty of Agriculture, Islamic Azad University, Chalous, Iran.

²Reference Mycoplasma Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran.

³Clinical Sciences Department, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Campus, Tehran, Iran.

⁴Clinical Sciences Department, Faculty of Veterinary Science, University of Tehran, Iran.

*Corresponding author. E-mail: erfsat@yahoo.com.

Accepted 3 January, 2012

   Abstract

Mycoplasma synoviae (MS) is one of the important pathogens in chicken and turkey which cause great economic losses in poultry industry. M. synoviae has one serotype but there is heterogeneity among MS strains. The aim of this study was to analyze the DNA sequence of Mycoplasma synoviae isolates from Mazandran province poultry flocks of Iran and compare them with MS from other countries. For this purpose, 32 samples were collected from choanal cleft, trachea and air sac of flocks with respiratory symptom and cultured in specific PPLO medium. Ten out of the 32 samples were positive in culture and reacted with specific antisera. The 10 positive samples were subsequently used for molecular study. The PCR products containing 16s rRNA genome were generated with specific primers and then sequenced. Multiple alignment and phylogenic analysis of DNA sequences was performed with DNASTAR program. Alignment and comparison of the sequences in DANSTAR program revealed that 7 of 10 isolate are quite similar and 3 other isolate are different from group one. Phylogenic analysis of M. synoviae isolated from Mazandran province and 21 M. synoviae from the entire world revealed low homology among most of them. However, some of the Mazandran isolates showed high homology with M. synoviae isolated from Brasilia. Overall, these results revealed the different molecular structure and heterogeneity among M. synoviae isolated from Mazandran province in this study.

Key words: Mycoplasma sinoviae, sequence, phylogenetic, poultry, Mazandran.