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Production
of extracellular protease in submerged fermentation by
Bacillus
licheniformis
ATCC 12759
Nurullah Akcan
Health High School, Siirt University, TR-56100 Siirt –
TURKEY. E-mail:
nurullah.akcan@gmail.com. Tel: +90 484 223 10 56.
Fax:+90 484 223 51 56.
Abbreviations: SmF,
Submerged fermentation; SD, standard deviation.
Accepted 7 October, 2011 |
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The various nutrients belonging to carbon, nitrogen and
amino acid sources, were investigated in terms of their
effect on the production of extracellular protease by
Bacillus licheniformis
ATCC 12759.
Comparison with the control in media containing all the
simple sugars
resulted in a decrease in
proteolytic activity,
while there was significant increase in enzyme yield in the
case of the supplementation complex carbon source such as
wheat flour and rice flour. Urea and
sodium nitrate
were the best organic and inorganic nitrogen sources,
respectively. Among the amino acid sources tested,
L-phenylalanine, L-cysteine, glycine and L-valine favored
the production, respectively. FeSO4, ZnSO4
and CuSO4 completely repressed protease
production. Maximum protease production (10738.2±44.2 U/mg)
was obtained in a medium containing 0.1% MgSO4 in
24 h 37°C.
Keywords:
Bacillus licheniformis
ATCC 12759, protease,
submerged fermentation, enzyme production |
