Full Length Research Paper

GFLV coat protein constructs based on local isolates from the Czech Republic

Josef Vlasák¹*, Jindřich Břiza¹, Hana Niedermeierová¹, Daniela Pavingerová¹, Aleš Eichmeier², Miroslav Baránek² And Miroslav Pidra²

¹Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences

Of the Czech Republic, České Budějovice, Czech Republic.

²Faculty of Horticulture in Lednice, Mendeleum – Institute of Genetics,

Mendel University in Brno, Lednice, Czech Republic.

*Corresponding author. E-mail: vlasak@umbr.cas.cz. Tel: +420 387 775 514. Fax: +420 385 310 356.

Abbreviations: GFLV, Grapevine fanleaf virus; CP, coat protein; GMO, genetically modified organism.

Accepted 11 November, 2011

   Abstract

Coat protein genes of grapevine fanleaf virus local strains isolated in South-Moravia, Czech Republic were sequenced, and artificial coat protein gene was designed and synthesized. It retains typical sequence features of local strains but is free of mRNA destabilizing sequences. Three variants of the synthetic gene were cloned into Agrobacterium plant expression vector and their function was tested after potato protoplasts transformation, assaying transient mRNA and coat protein production.

Key words: Grapevine fanleaf virus, Vitis vinifera, sequence, protoplast expression, resistance.