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Effects of
space flight factors on genetic diversity of Buchloe
dactyloides seeds
Zhang Bao-Yi, Wei Xiao-Lan, Yang Fu-Yu
and Zhang Yun-Wei*
Institute of Grassland Science, China Agricultural
University, Beijing, 100094, China.
*Corresponding author. E-mail:
zywei@126.com. Tel:
+86-010-62733409
Accepted 1 September, 2011 |
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The objective of this research was to investigate the
effects of space flight factors on Buchloe dactyloides “Jingyin
No.3” seeds. After the retrieval, basic turf characters of
plants were tested. Among the 100 plants tested, 21 showed
great change on phenotype characters, including leaf blade
length and width, height, stem diameter, number of tillers,
number and length of stolon, length of stolon inter node,
leaf color and extent of leaf turning yellow.
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primers were screened in inter-simple sequence repeats (ISSR)
analysis to evaluate DNA variation between mutations and
their ground controls. Results show that 15.6 reliable bands
were generated by 7 primers, of which 12.9 (80.9%) were
polymorphic. Based on the study, we can conclude that the
space flight factors could induce inheritable mutagenic
changes on B. dactyloides seeds, and do further
research to demonstrate these changes in genetic material of
the mutants.
Key words:
Genetic diversity, Buchloe dactyloides, spaceflight,
inter-simple sequence repeats. |
