Differentially expressed genes in white egg 2 mutant of
silkworm, Bombyx mori, at early embryo development
Yadong Wei2,3, Dingguo Xia2,3, Yeshun
Zhang2,3, Bo Deng2, Ziyu Liu2,
Jiekui Zhang2 and Chengfu Lou1
1College of Animal
Sciences, Zhejiang University, Hangzhou 310029, Zhejiang
Province, PR China.
2Jiangsu University of
Science and Technology, Zhenjiang, 212018, Jiangsu Province,
Institute, Chinese Academy of Agricultural Sciences,
Zhenjiang, 212018, Jiangsu Province, PR China.
*Corresponding author. E-mail:
Tel: +8651185616539. Fax: +8651185628183.
Accepted 26 September, 2011
White egg 2 is one of white egg mutants in silkworm, whose
molecular mechanism remains unknown so far. In order to
obtain an overall view on gene expression profiles at early
embryo development stages, the white egg 2 near-isogenic
line was constructed and the whole-genome of silkworm
microarray system containing 21375 predicted genes from the
silkworm whole genome sequence was employed to investigate
gene expression profiles at 0, 24 and 48 h post oviposition
between white egg 2 mutant and normal black egg strain. At
24 h post oviposition, 49 genes exhibited at least 2.0 fold
differences at expression level, including 24 up-regulated
genes and 25 down-regulated genes while at 48 h post
oviposition, 52 genes, including 23 up-regulated genes and
29 down-regulated genes were expressed differentially over
2.0 change fold. Kyoto Encyclopedia of Genes and Genomes (KEGG)
pathway analysis indicated that nine differentially
expression genes were involved in nine significant (p<0.05)
pathways at 24 h post oviposition and 24 significant
pathways at 48 h post oviposition, respectively. These
pathways were related to amino acid metabolism, sugar
metabolism, and series of major physiological metabolism.
Our results hopefully shed light on the further study of
molecular mechanism of white egg 2 mutant.
white egg 2 mutant, microarray, embryo, differentially