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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 10 No. 36

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  Search Pubmed for articles by:

 
 Momtaz H

   Safari S

 

 
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African Journal of Biotechnology Vol. 10 (36), pp. 6857-6862, 18 July, 2011

DOI: 10.5897/AJB11.513

ISSN 1684-5315 © 2011 Academic Journals  

 

Full Length Research Paper

 

Comparisons of competitive enzyme-linked immunosorbent assay and one step RT-PCR tests for the detection of Bluetongue virus in south west of Iran

 

Hassan Momtaz1*, Shahin Nejat2, Negar Souod3, Monochehr Momeni4 and Sohrab Safari4

 

1Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord   Branch, Shahrekord-Iran.

2Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord-Iran.

3Master of Science of Microbiology, Member of young researchers club, Islamic Azad University, Jahrom Branch, Jahrom-Iran.

4Research Center of Biotechnology, Islamic Azad University- Shahrekord Branch- Shahrekord- Iran.

 

*Corresponding author. E-mail: hamomtaz@iaushk.ac.ir or hamomtaz@yahoo.com. Tel/Fax: +983813361064.

 

Abbreviations: C-ELISA, Competitive enzyme-linked immunosorbent assay; RT-PCR, reverse transcriptas- polymerase chain reaction; BTV, Bluetongue virus.

 

Accepted 9 June, 2011

 

   Abstract

 

Bluetongue is a noncontagious, arthropod-borne viral disease of both domestic and wild ruminants. Bluetongue virus (BTV) is the type of species of the genus Orbivirus within the family Reoviridae. BTV is endemic in some areas with cattle and wild ruminants serving as reservoirs for the virus. Clinical symptoms are often seen in sheep. There are several methods for the detection of Bluetongue virus, among them the molecular technique like RT-PCR is considered as the most sensitive and reliable one. The aim of this study was to comprise competitive enzyme-linked immunosorbent assay (C-ELISA) with one step RT-PCR test for the detection of BTV in sheep. A total of 770 blood samples were obtained from sheep (265 serum positive samples and 505 serum negative samples in C-ELISA). According to our data, out of the 265 serum positive samples in ELISA test, 234 were positive in RT-PCR assay whereas all serum negative samples were negative in RT-PCR experiment. According to the results, the PCR assay was more sensitive and reliable than ELISA technique for the diagnosis of Bluetongue virus.

 

Key words: Bluetongue virus, C-ELISA, RT- PCR, Sheep, Iran.

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