Full Length Research Paper

Genetic diversity of Pogonatherum paniceum (Lam.) Hack in Southwest China revealed by ISSR

Zeng-Bin Luo^1#, Guo-Qing Zhuang^2#, Dan-Wei Ma³ and Fang Chen¹*

¹College of Life Science, Sichuan University, Chengdu 610064, China.

²Sichuan Academy of Forestry, Chengdu 610081,China.

³College of Life Science, Sichuan Normal University, Chengdu 610066, China.

*Corresponding author. E-mail: lifeisword@sina.com.cn. Tel/Fax: 86-28-8540-3278.

Abbreviations: ISSR, inter-simple sequence repeats; PPB, percentage of polymorphic bands; PCR, polymerase chain reaction; UPGMA, unweighted pair group method with arithmetic mean; TFPGA, tools for population genetic analysis; CTAB, cetyltrimethyl ammonium bromide; AFLP, amplified fragment length polymorphism.

^#These authors contributed equally to this paper

Accepted 26 November, 2009

   Abstract

Inter-simple sequence repeats markers were used to estimate the genetic diversity of Pogonatherum paniceum (Lam.) Hack. from Sichuan Province, Yunnan Province, Chongqing City and Guangxi Zhuang  Autonomous Region in China. 100 primers were carried out on 22 wild populations, 14 could produce highly reproducible inter-simple sequence repeats markers′ bands. Out of the 239 discernable DNA fragments, 227 were polymorphic. The percentage of polymorphic bands was 94.98% at the species level. Nei’s gene diversity and Shannon information index were 0.312 and 0.471, respectively. This indicated that the genetic diversity of P. paniceum (Lam.) Hack. was low. The values of genetic identity ranged from 0.548 to 0.820 with a mean of 0.673. Nei’s genetic distance between 22 populations ranged from 0.198 to 0.601. Unweighted pair group method with arithmetic mean cluster analysis based on Nei’s genetic distance showed that most populations were positioned into the relevant areas. Significant correlation between genetic and geographic altitude distances among populations was found by Mantel test. The high score of percentage of polymorphic bands might be caused by low frequent polymorphism distributed in different populations.

Key words: P. paniceum (Lam.) Hack., ISSR, genetic diversity, southwest China.