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Optimization
of lipase production by Staphylococcus sp. Lp12
Pallavi Pogaku*,
A. Suresh, P. Srinivas and S. Ram Reddy
Department of Microbiology, Kakatiya
University, Warangal-506009. A. P. India.
*Corresponding author. E-mail:
Pallavi.pogaku@yahoo.com.
Accepted 30 December, 2009 |
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A bacterial strain isolated from an oil contaminated soil,
identified as Staphylococcus sp. Lp12 was screened
for lipase activity on tributyrin agar and spirit blue agar
medium. Maximum lipase production was observed at 48 h of
growth (3.5 Eu/ml). Peptone was found to be as an ideal
nitrogen source for production at a concentration of 1.0%
(4.25 Eu/ml). Addition of any nitrogen source other than
peptone to the medium resulted in a significant reduction of
enzyme production. Lower lipase production was noted when an
inorganic nitrogen source was used as the sole nitrogen
source. Starch was used as a major carbon source for optimum
production of lipase (4.25 Eu/ml) at a concentration of
1.5%. Of the natural oils, olive oil was able to induce more
lipase (4.25 Eu/ml) rather than the oils like groundnut,
coconut, castor oils. Basal medium containing tween 80
enhanced lipase production to a significant level. The pH 8
and temperature 45°C were found to be ideal pH and
temperature for optimum production of lipase by this strain.
Key words:
Staphylococcus sp. Lp12, lipase, tributyrin,
spirit blue agar. |
