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Effects of
salinity on sucrose metabolism during tomato fruit
development
Shaowei Lu,
Tianlai Li* and Jing Jiang
The Key Laboratory of Protected
Horticulture of Liaoning Province, College of
Horticulture, Shenyang Agricultural University, Shenyang
110161, China.
*Corresponding author. E-mail:
tianlaili@126.com.
Abbreviations: HLPC,
High-performance liquid chromatograph; cDNA,
complementary; PCR, polymerase; SS, sucrose
synthase; AI, soluble acid invertase; SAI,
insoluble acid invertase; NI, neutral invertase;
SPS, sucrose phosphate synthase; DAA, days after
anthesis.
Accepted 7 January, 2010 |
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The objective of this study was to investigate the effects
of salt stress on content of carbohydrate and activity of
sucrose-metabolizing enzyme and gene expression patterns and
to provide a new evidence for tolerating salt stress of
cultivated tomato. The related enzymes’ activities of
sucrose metabolism including invertase,
sucrose synthase and sucrose phosphate synthase and the
expression of acid invertase and sucrose synthase mRNAs were
determined. The results indicated that hexoses (fructose and
glucose) accumulated to higher levels and the content of
sucrose and starch is lower in mature fruit under salt
stress treatments; the salinity can maintain or enhance the
invertase activities of tomato fruit in a long period of
time (20 - 60 days after anthesis) and elevate the
expression of acid invertase mRNA. Salinity could also
regulate the sucrose synthase activity by controlling its
gene expression. But the effects of salinity treatment on
sucrose phosphate synthase activities were weak under the
condition of salt stress. It showed that salinity could
regulate the activity of sucrose-metabolizing enzymes by
controlling its gene expression under salt stress.
Key words:
Lycopersicon esculentum, salinity, sucrose metabolism,
gene expression. |
