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Molecular
cloning and characterization of a novel expressed sequence
tag (EST) associated with fecundity in goats
Y. Q. He*,
J. Kong, X. K. Ma, C. X. Zhang, X. Y. Liu and H. Q. Chen
Institute of Life Sciences, Jiangsu University, 301# Xuefu
Road, Zhenjiang, 212013, P.R. China.
*Corresponding author. E-mail:
yqhe@ujs.edu.cn. Tel:
+86-511-88791702.
Abbreviations:
EST,
expressed sequence tag;
cDNA,
complementary DNA; DNase, deoxyribonuclease; RNase,
ribonuclease; dNTP, deoxyribonucleoside
5’-triphosphate; RT, reverse transcription; PCR,
polymerase chain reaction; RT-PCR, reverse
transcriptase- PCR; PAR-2, protease-activated
receptor-2; CNS, central nervous systems; Mel(1a),
melatonin receptor 1a; Mel(1b), melatonin receptor
1b; EDTA, ethylene diamine tetraacetic acid;
G-protein, guanosine nucleotide-binding protein; DD-PCR,
differential display- PCR; DDRT-PCR, differential
display RT-PCR; RACE, rapid amplification of cDNA
ends.
Accepted 19 January, 2010 |
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To screen the genes controlling the fecundity traits in
goats, a DDRT-PCR technique was applied. We found a new EST
which highly expressed in Chinese native prolific goat
breed, Haimen goats. There exists a difference of EST
expression level between the prolific and non-prolific goat
breed, indicating EST might associate to fecundity in goats.
A full-length cDNA with 2253 base pairs was obtained by the
3’- and 5’-RACE method based on the EST sequence encoding a
protein segment of 201 amino acid residues. Tissue specific
distribution and sequence analysis implicated the likely
involvement of EST in the regulation of the hormones related
to fecundity.
Key words:
Goat, expressed sequence tag, reproduction. |
