of eleven Kobresia accessions from the Tibetan
Peizhi Yang1, Hongmei Zheng1,
Steven Larson2, Yanjun Miao1 and
of Grassland Science, College of Animal Science and
Technology, Northwest Agriculture and Forestry University,
Yangling, Shaanxi, 712100, P.R. China.
and Range Research Laboratory, Utah State University,
USDA-ARS, Logan, Utah, 84322-6300, USA.
*Corresponding author. E-mail:
Tel: +86 13709124728.
Random amplified polymorpfhic DNA; AFLP, amplified
fragment length polymorphism; GIS, geographic
cetyl trimethy-lammonium bromide; PCR,
polymerase chain reaction; PIC, polymorphism
information content; ITS, internal transcribed
spacers; PPB, percentage of polymorphic bands.
14 May, 2010
In the past, identification of the
genus Kobresia was mostly dependent on morphological
characteristics. This study used random amplified
polymorphic DNA (RAPD), sequences of nrDNA ITS, cpDNA trnT-L-F
spacer and cpDNA ndhF to assess the phylogenetic
relationships among the accessions of Kobresia plants
collected from the Tibetan plateau. In the dendrograms,
Kobresia macrentha (L) species formed a separate clade
suggesting a remote relationship with other accessions.
These trees showed that species found in similar habitats or
having similar adaptations tended to cluster together. Thus,
the genetic variation and adaptation seen in these
Kobresia accessions may be due to their remote
geographic and high altitudinal position in the Tibetan
plateau. This study highlights the importance of molecular
analysis in understanding the genetic diversity and
structure of Kobresia accessions, and contributes to
the knowledge of conservation of genetic resources.
Kobresia, phylogenetic relationships, random
amplified polymorphic DNA, Tibetan plateau.