Full Length Research Paper

Amplified fragment length polymorphism (AFLP) and genealogy analysis of the introgressed lines from Gossypium hirsutum × Gossypium barbadense varieties

Zhao-hui Liu^1,2, Yong Zhang^1,3*, Jinhua Peng¹, Kejun Deng¹, Shunwen Dong² and Zhenglong Ren^1,3*

¹School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu, 610054, P. R. China.

²Industrial Crop Breeding and Cultivation Institute, Sichuan Academy of Agriculture Sciences, Jianyang 641400, P. R. China.

³Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Ya’an, 625014, P. R. China.

*Corresponding author. E-mail: zhangyong916@uestc.edu.cn. Tel: 86-28-83203928. renzl@uestc.edu.cn Tel: 86-835-2882123

Abbreviations: AFLP, Amplified fragment length polymorphism; PCR, polymerase chain reaction; SSR, simple sequence repeats; RAPD, amplified polymorphic DNA.

Accepted 21 May, 2010

   Abstract

In this study, amplified fragment length polymorphism (AFLP) technique was used to analyze the genealogical relationship of 7 introgressed cotton varieties from the hybridization of upland (Gossypium hirsutum L) and sea-island cottons (Gossypium barbadense L). Ten pairs of primer combinations with high polymorphism and resolution were selected from 64 primers. These 10 primer combinations resulted in a total of 480 bands, of which 374 bands (77.9%) were polymorphic and 51 bands (10.6%) were specific. Cluster analyses showed that 7 varieties of cottons were divided into two groups. Our results suggested that interspecific hybridization is feasible to broaden germplasm of upland cotton and AFLP technique could be applied for the identification of variety purity and genealogical relationship.

Key words: Cotton, introgressed line, AFLP, genealogical analysis.