Amplified fragment length
polymorphism (AFLP) and genealogy analysis of the
introgressed lines from Gossypium hirsutum ×
Gossypium barbadense varieties
Zhao-hui Liu1,2, Yong Zhang1,3*,
Jinhua Peng1, Kejun Deng1, Shunwen
Dong2 and Zhenglong Ren1,3*
of Life Sciences and Technology, University of Electronic
Science and Technology of China, Chendu, 610054, P. R.
Crop Breeding and Cultivation Institute, Sichuan Academy of
Agriculture Sciences, Jianyang 641400, P. R. China.
Laboratory of Plant Breeding and Genetics, Sichuan
Agricultural University, Ya’an, 625014, P. R. China.
*Corresponding author. E-mail:
firstname.lastname@example.org. Tel: 86-28-83203928.
Amplified fragment length polymorphism; PCR,
polymerase chain reaction; SSR, simple sequence
repeats; RAPD, amplified polymorphic DNA.
In this study, amplified fragment length polymorphism (AFLP)
technique was used to analyze the genealogical relationship
of 7 introgressed cotton varieties from the hybridization of
and sea-island cottons (Gossypium
Ten pairs of primer combinations with high polymorphism and
resolution were selected from 64 primers. These 10 primer
combinations resulted in a total of 480 bands, of which 374
bands (77.9%) were polymorphic and 51 bands (10.6%) were
specific. Cluster analyses showed that 7 varieties of
cottons were divided into two groups. Our results suggested
that interspecific hybridization is feasible to broaden
germplasm of upland cotton and AFLP technique could be
applied for the identification of variety purity and
Cotton, introgressed line, AFLP, genealogical analysis.