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Molecular cloning and
functional analysis of the gene encoding geranylgeranyl
diphosphate synthase
from Jatropha curcas
Juan Lin*, YuanJie Jin,
Xin Zhou and JingYa Wang
State Key
Laboratory of Genetic Engineering, Institute of Plant
Biology, School of Life Sciences, Fudan University,
Shanghai, People’s Republic of China.
*Corresponding author. E-mail:
linjuan@fudan.edu.cn.
Accepted 1
April, 2010 |
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Geranylgeranyl diphosphate (GGPP) synthase (GGPPS, EC:
2.5.1.29) catalyzes the condensation of isopentenyl
diphosphate
(IPP) with allylic diphosphates to yield (all-E)-GGPP.
GGPP is one of the key precursors in the biosynthesis
of biologically significant isoprenoid compounds. Here we
report for the first time the cloning of a full-length cDNA
encoding GGPPS (Jc-GGPPS) from Jatropha curcas L. The
full-length cDNA was 1414 base pair (bp), with an 1110-bp
open reading frame (ORF) encoding a 370-amino-acids
polypeptide. Bioinformatic analysis revealed that Jc-GGPPS
is a member of the polyprenyltransferases with two highly
conserved aspartate-rich motifs, and showed high homology to
other plant GGPPSs. Phylogenetic tree analysis indicated
that plant GGPPSs could be classified into two groups,
angiosperm and gymnosperm, while Jc-GGPPS had closer
relationship with angiosperm plant GGPPSs. Functional
analysis of Jc-GGPPS in GGPPS-deficient mutant plasmid pAC-BETA
(³ crtE) demonstrated that Jc-GGPPS mediated the
biosynthesis of carotenoid and provided the general
precursor for diterpenes biosynthesis.
Key words: Jatropha curcas L., geranylgeranyl diphosphate
synthase, rapid amplification of cDNA ends, genetic
complementation. |
